I am currently planning to use vcDIO construct and I saw Nature Method paper:

Targeting cells with single vectors using multiple-feature Boolean logic

and plasmid map deposited on Addgene.

One thing confuses me is the sequence of vloxp and vlox2272, they are not intersected like creDIO sequence(loxp-lox2272-loxp-lox2272);

but aligned like this: vloxp-vlox2272-vlox2272-vloxp.