When detecting the activated microglia, studied commonly use the increased expression level of IBA-1 in IF or IHC to show the activation of microglia, since the proliferation of microglia is dependent of activation of microglia, why not use calculate the number of activated microglia or use both which seems more convincing.
Dear Su, I agree with you that signal intensity in IF is not the best way, and counting the positive cells is more reliable. Of course, counting the total number of cells can also be challenging sometimes.
Hello,
Microglia activation does not always means that the microglial cells will proliferate. In acute activation, microglia will often switch phenotype without dividing. Upon activation microglia will express new markers but will also upregulate markers such as Iba1 or CD11b.
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