Why turkey or sheep red blood cells used in hemagglutination assay?

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How to calculate self-ignition temperature of CH4/H2 and C3H8/H2 mixture?

how to calculate the self ignition temperature for hydrogen enriched fuel ?

24 April 2022 216 4 View

Why the level of free ubiquitin is degrading in my in-vitro ubiquitylation reaction?

I am doing in-vitro ubiquitylation reactions where I am treating with DUB's to cleave di-ubiquitin and tri-ubiquitin, but surprisingly level of free ubiquitin is also degrading instead the fact...

05 December 2021 6,288 1 View

Dear researchers, I want to convert cantera file(.cti) to chemkin mechanism (.inp) file for the Z77 kerosene mechanism file. Please help me on this?

Z77 mechanism for kerosene combustion involves 77 reactions. However, the file is available in cantera format. Please convert in to chemkin format.I have attached the file in this question.

01 September 2020 2,728 3 View

What are the practical applications of elastic foundations?

Hello everyone!!! I am very curious to know about the real life applications of various elastic foundations such as Winkler elastic foundation, Pasternak elastic foundation etc. Thank you in advance

10 April 2019 6,156 7 View

How to calculate chemical reaction time in fluent?

Dear researchers, I want to calculate the chemical time scale in combustion process using fluent as Damkohler number suggest the ratio of Flow time to chemical...

27 December 2018 7,741 2 View

Why am I getting frequency parameters as complex numbers and complex conjugates? What does it mean?

Hi all!!! I am trying to obtain frequency parameters of one dimensional nano-structure which is placed on an elastic foundation by using MATLAB code. But I am getting complex numbers (purely...

24 December 2018 3,536 3 View

How to get Damkohler number contour for combustion problem in fluent?

Dear Friends, I am working in non-premixed MILD combustion.In fluent contour of Damkohler number is not available.So, my question is how to activate contour of Damkohler...

23 December 2018 2,320 2 View

After what tonnage of rolling,the backup rolls(3.5% Cr Forged steel) of finishing stands of 4 high hot strip mill sholud be changed

Back up rolls of hot strip mill need to be changed.After rolling of how much tons of steel they should be changed?

23 June 2017 935 1 View

What are the probable reasons for non-superconductivity in an Ex-situ processed MgB2 wire?

I prepared some MgB2 strand sample in lab scale to characterize the powders. Strands prepared through In-situ route are showing better result but at the same time Ex-situ processed wires are not...

11 October 2015 9,123 6 View

What is the best software available for XRD data analysis?

I tried fullprof, but I do not know where I should start. How can I use fullprof? Is there any training program or manual available?

23 April 2014 2,317 14 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

How high should the remuneration be for test subjects in psychological research projects in Germany?

I am working on a project proposal (DFG project in Germany). I have the problem that I want to calculate how much funds I have to request for my test subjects (e.g., for qualitative interview of...

18 July 2024 1,690 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Is there any one working on Sister chromatid exchange and micronucleus assays in India?

I am working on SCE and micronucleus assay. I completed around 30 samples of SCE and MN but suddenly the results get stopped and its been above 1 year struggling with the cell culture experiment....

02 July 2024 7,649 3 View

Help to isolate Ralstonia from plant tissue?

I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...

02 July 2024 9,807 3 View

How do you bleed HIV-infected humanized mice?

Hello everyone, I usually use the saphenous vein to collect blood from HIV-infected mice (for viral load, CD4/CD8, etc.). However, I would like to know what methods other researchers are using to...

27 June 2024 7,707 2 View