I try to optimize mobile phase solvents for gallic acid FROM PLANT EXTRACT without toluene using TLC?
Please open this data base, I think you can have the answer:
http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm?method=query
Please refer this article: http://www.vitis-vea.de/admin/volltext/e049135.pdf
You can use this solvents system for TLC, chloroform : ethyl acetate: formic acid, 5:4:1 v/v/v ( Silicagel GF254) , detection UV 254 and FeCl3 (2% in ethanol). Good luck.
The h-index is a widely used metric for measuring an individual's research output. While it is a useful tool, it should not be the sole determinant of a researcher's quality that I think about it,...
21 March 2023 1,192 5 View
Hello Im trying to extract the Quality factor from transient response of multiple LDOs connected to a PDN. The circuit is very complex in the sense that it's very big. So I have to make use of...
21 June 2022 6,493 2 View
Hello, I am performing an immunofluorescence TUNEL assay using an abcam kit (ab66110) on frozen tissues of kidney mice using the following protocol from abcam (frozen tissues section)...
14 April 2021 446 2 View
I have six dependent variables (continious) and two independent ones (one ordinal and one continious). My sample size is only 14 participants. I got some correction that Pearson Correlation...
29 June 2020 5,851 7 View
I used a 330 bp long RNA sequence to predict its secondary structures using (RNAstructure online tool) and got 20 different structures of energy values between 105 and 107 Kcal/mol. Now I don't...
13 June 2020 6,581 0 View
As we know publishing research or experimental papers sometimes so difficult, let's see important steps and basic concepts to verify that.
21 May 2020 772 1 View
earnings management and cost of capital
15 April 2020 6,262 1 View
Does anyone knows and could give us the steps for how can make SiO2 nanoparticles as a coating on BFRP bar
19 March 2020 10,096 8 View
I would like to know the international and regional journals for publication I want to know the websites of the magazines to communicate with. I have a legal research (the field of labor law) I...
22 January 2019 8,639 5 View
Hello, I am trying to measure the enzymatic activity of SOD in my cell lysate via the xanthine oxidase and NBT method. However i am not able to find a clear protocol for that. Can anyone help me ?
06 December 2018 8,361 4 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
How can I measure the thickness of thin film by Uv-Vis?
06 August 2024 7,810 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View