Can anyone provide reference materials for Deformation Based Morphometry(DBM) ?

I heard that this parameter (DBM) is well known from continuum mechanics and is usually used for the analysis of volume changes in flowing liquids or gases. I am seeking some theories of how does...

09 October 2018 5,550 2 View

Why people use higher smoothing kernels for surface based measurements of brain?

Generally for tissue volumes(GM, WM and DBM) we use 8-12 FWHM for smoothing. But in some softwares recommend 15 mm for cortical thickness and 20-25 mm for all other indexes (gyrification, cortical...

03 April 2018 5,661 3 View

Does ICA meaningful for structural (T1W) brain data?

Currently ICA is applied for functional data. But...I feel this could be little debating.

31 December 2017 3,691 4 View

How to obtain individual subject's ICAs using group ICAs?

I have obtaind 9 group ICs using GIFT. Now I want to extract each ICs of each subject using group ICs. Subject matrx(contains voxels of each subject)=Mixing matrix(contains weight of each...

31 December 2017 1,722 2 View

How to do searchlight MVPA for surface based measurements of Brain?

I have done searchlight MVPA for GM matter volumes and deformation based measurements sucessfully. But I have converted .gii files of cortical thickness to .mat and awaiting to do searchlight...

11 December 2017 4,545 0 View

What are the best requirements/specs for an efficient image processing laptop?

I am working on Neuroimaging...

10 November 2016 7,932 0 View

Can localize or map microorganism (Ex- Mycobacterium Tuberculosis) distribution of TB patients using radio-pharmaceutical?

08 September 2015 8,747 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View