Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

What are the different subclasses in intermediate seeds?

I am currently working on an endemic tree species, that produces mature seeds (moisture content 51%) which shows both vivipary (30% of total harvested seeds from a tree) and dormancy (~40%) of...

20 January 2021 2,670 3 View

Do you can put again my publications online?

Dear All, Please I has accidentaly removed 22 publications from RG my profile in the mail that you send me today, please put again those publicatios in my profile. Best regards,

13 December 2020 9,981 4 View

Adhesion energy (MOF-water) >> cohesion energy (MOFs) results higher uptake- how can I prove it thermodynamically?

In a surface energy analysis, we have found a very interesting result- the cohesive energy of aluminum fumarate is lower than the adhesive energy of aluminum fumarate-water pair. Also, the uptake...

05 November 2020 4,292 2 View

How can one automatically extract drainage basin values from a DEM following Strahler stream ordering approach?

Analysing hydrologically corrected DEM avails an opportunity to calculate morphometric indices such as drainage basin density(DD) (which takes manual approach). Can not DD and others be extracted...

07 October 2020 3,893 3 View

How to hydroxylate the surface of bismuth telluride nanoparticles?

Would it be possible to add -OH functional groups to Bi2Te3 nanoparticles?

07 October 2020 6,560 3 View

What is the chemical stability of bismuth telluride either in bulk or nanoparticle form (e.g., nanowires, platelets, etc)?

I am interested in post-synthethic methods of this material, and would like to know the chemical stability of Bi3Te2 (particularly nanotubes) in water, acid o alkaline media. Any literature...

03 October 2020 2,743 3 View

Does reducing the brain neuronal network as an inevitable aging event serve to consolidate memory tracking?

07 September 2020 6,188 2 View

What are all the criteria for the occurrence of Lunar Eclipse?

I have read the maximum limit of Lunar Eclipse is 12.2 degrees of arc (i.e. the difference in the longitude between Moon and any one node of the Moon). However on on June 5th 2020, a partial Lunar...

27 August 2020 986 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View