Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

How long does it take for MCF-7 cancer cells to adhere to the microfluidic surface? Is 24 hours enough?

Hello everyone. We have cultured MCF-7 cancer cells in microfluidics several times and incubated them for 24 hours, but as the nanomaterial passed through the cells, the cells moved and no cell...

05 September 2020 7,292 3 View

How can I make DMEM F12 with 30mM glucose concentration for induction of stress oxidative in HUVEC cells?

Hi dear researchers, DMEM F12 glucose concentration is 17.5mM, I added D_glucose in basal cell culture until reached 30mM, but HUVEC cells had better grown than control group after 48 hours. I...

06 April 2018 8,650 1 View

Clustering network vehiculer in ns2?

I need to compare my work with the Reservation-Based (RB)code that described in the "Offloading Floating Car data" article. Can anyone give me the its code? I work with ns2 simulator

06 September 2017 3,631 1 View

What is the longest lifetime reported for electron and holes in perovskite solar cells to date?

When these materials get excited with light, electron and holes are generated but it is important for them to hang arround for a while before they recombine so that more photocurrent will be...

26 August 2017 9,161 11 View

What is the density of poly vinyl alcohol (SB220)?

Density of PVA (SB220)

06 February 2017 8,921 5 View

How can i detect fire using SAR (RADARSAT-2) backscatter and optical data?

I would detect fire, insect and deforestation (logging and building) disturbance using SAR backscatter and Optical data for Hyrcnian forest in the Iran.

02 October 2016 2,129 4 View

How to prevent/reduce hysteresis effect in dye sensitized solar cells (DSSCs) / perovskite solar cells?

I have read that one of the major challenges in solar cells applications is reducing Hysteresis. First off, can anyone give an explanation of this phenomenon? Secondly, how to face this...

27 July 2016 8,620 5 View

Can we study Pests and dieback using L-band PALSAR data?

12 July 2016 6,369 2 View

What is the best method for miRNA isolation from rice roots?

Please help me to choose a suitable method or kit for miRNA isolation from rice roots towards sequencing (high throughput data). I research on rice root tips under drought condition. Thank you in...

28 April 2016 2,730 1 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

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01 March 2021 3,905 5 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View