I extracted DNA from tomato leaves by CTAB method. After precipitation by isopropanol, I got DNA pellet black in color. What would be the probable reason of pellet getting black and what is the solution to remove black color from pellet?
The black color of DNA in tomato is normal ,no need to do any thing to remove the color go on in your work
@Thaera Alrawi Thank you so much for answer. could you please tell me the reason?
Ayesha Wadood The pellet is black due to polyphenols. They can affect quality of PCR and/or restriction, and it is better to purify DNA using specific methods, following:
Peterson DG, Boehm KS, Stack SM. Isolation of milligram quantities of nuclear DNA from tomato (Lycopersicon esculentum), a plant containing high levels of polyphenolic compounds. Plant Molecular Biology Reporter. 1997 Jun;15(2):148-53.
Couch JA, Fritz PJ. Isolation of DNA from plants high in polyphenolics. Plant Molecular Biology Reporter. 1990 Feb;8(1):8-12.
Porebski S, Bailey LG, Baum BR. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant molecular biology reporter. 1997 Mar;15(1):8-15.
You can purify your DNA by the PEG purification method or by the ethanol precipitation method, both are easily available methods. Sometimes, polyphenol or other contaminants are the reason behind this....
Solanaceae family contain more chlorophyll that's why ethanol washing 3-4times needed...
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