Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Distortion problem in Abaqus explicit?

Dear all. I'm modeling the RC column with cyclic displacement by Abaqus-Explicit. The concrete model used Concrete Plastic Damage model. However I'm facing with a error about the excessive...

01 February 2021 7,726 1 View

How to intepret data with significant ANOVA but not significant Tukey test?

I have this set of data: Treatment 1: 30.5; 34.5; 24.4 Treatment 2: 24.8; 20.8; 16.8 Treatment 3: 19.1; 21.4; 21.0 Treatment 4: 22.3; 26.1; 27.1...

25 November 2020 920 4 View

Acesulfame Potassium and Acesulfame and Conversion?

Dear to whom it may concern, Based on ChemAxon, I calculated pKa values of Acesulfame as follows: 1. pKa (Strongest Acidic): 3.02 2. pKa (Strongest Basic): -6 Because I would like to convert...

25 November 2020 4,577 3 View

How to perform Cluster analysis for segmentation with two latent factors?

Hello, My name is Elena and working on cluster analysis for respondents segmentation based on two latent factors. I wonder whether I am doing the right way. I have 400 respondents answering on 2...

19 October 2020 1,697 3 View

How to avoid protein precipitation during Ni-NTA binding step?

Hi everyone, I am purifying an 18 kDa protein (his-tag). The protein expresses very well and I am able to get a clean 18kDa band in SDS Page gel after purification (attached picture). However,...

11 October 2020 3,189 6 View

Difference between Porous Graphitic Carbon and Graphitized Carbon Black?

Dear to whom it may concern, May I ask you about the difference between porous graphitic carbon (PGC) and graphitized carbon black (GCB) in terms of surface properties, retention mechanisms, and...

10 October 2020 4,478 3 View

Carbonaceous Sorbent Materials and Force of Retention?

Dear to whom it may concern, I am investigating carbonaceous materials to treat aqueous samples but I have no knowledge of these materials. May I ask you some questions as follows? 1. Whether...

09 October 2020 3,716 1 View

How to invite call centers to participate in your study?

I'm conducting a job satisfaction study to predict telephone agents' turnover. I'm looking to increase participants for my study. How should I approach the directors of call centers to request...

20 September 2020 7,099 8 View

How do triploid acacia clones grow?

Some triploid acacia clones in Viet Nam have shown high potential in growth, disease resistance and good adaptation.

14 September 2020 1,983 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Does anybody know a public dataset of drop landing or jump landing maneuvers?

Dear all, In our project we would like to test different control strategies during drop landing compared to jump landing. After an intensive search I could not find a public dataset of drop...

09 February 2021 7,093 4 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

Which satellite image gives best results for Land Use land cover classification(specifically for built up regions) for Indian region ?

Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India

04 February 2021 8,632 8 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Is it possible to have a diffusion of oxygen from the glass substrate (SiO2) into the absorbent layer during annealing process?

02 February 2021 382 7 View