You must add a suitable fungicide working against Rhizopus to prevent its growth in the plates and allow the other fungi to grow.

Dear Dr. Stefan,

I have incubated the plates at 28 c only, for 7 days, and I found the plates covered with this famous hairy growth of Rhizopus

Dear Dr. Yahya,

I have not heared before about that fungicide which is specific for suppressing the growth of Rhizopus, if so, kindly give me its name.

Read this article Dr\ Marwa will greatly help you to stop growth of Rhizopus and Mucor using a specific medium called Dichloran-Rose Bengal Medium.

"Dichloran-Rose Bengal Medium for Enumeration and Isolation of Molds from Foods". Article Dichloran-Rose Bengal medium for enumeration of moulds from foods

Hopefully this may help you.

Dear, Mrawa,

Regarding your above comment "I have not heard before about that fungicide which is specific for suppressing the growth of Rhizopus, if so, kindly give me its name." , You can add Dichloran (2,6 dichloro-4-nitroaniline) to the medium to restrict the growth of Rhizopus, or use Dichloran-Rose Bengal Medium (DRBM).

With greetings.

Dear Dr. Adel Kamel Madbouly,

Thank you for suggesting this paper

Thank you Dr. Stefan for your concern and your answer

Dear Dr. Abdel-Azeem,

I will try that, I always trust your suggestions

Dear Dr. Salih,

It is the same medium suggested by Dr. Adel. Thank you

You can add the fungicide Dichloran to the basal medium with a concentration of 1mg Dichloran per 1 liter medium to prevent the growth of Rhizopus significantly especially, Rhizopus stolonifer.

The fungicide PCNB (Pentachloronitrobenzene) can be added to the basal medium to prevent Rhizopus growth and obtain a selective medium for preventing this fungus.

Dear, Dr. Ahmed,

I don't experiment this method (keeping the soil sample for two hours in deep freezer) before isolation and culturing. I should experiment it in the future. But what about the plant parts, if the sample was not a soil.

With regards

The fungicides: Difolatan, Mancozeb, Thiram, and Thiabendazole also can be added to the basal media with different concentrations according each one to prevent or restrict Rhizopus growth. But they are not in the same efficacy with Dichloran and PCNB.

Hi, Dr. Ahmed,

I ask, if we keep the soil sample in deep freezer, the other fungi may be killed by the freezing degree, then we can't isolate these fungi!!. What we do?

Kind regards