Why do T lymphocytes need to be activated prior to transfection?

So this is more of a curiosity of mine. Every protocol I find about the transfection of T-lymphocytes includes a two days stimulation prior to the transfection and I am wondering why that is....

29 July 2015 1,832 5 View

The modern system of international relations: unipolar or multipolar world?

Do you think that there is one center of power in the modern world, or are all countries equal in the international arena?

01 January 2021 7,026 2 View

Among all the environmental pollutants globally how much % out of 100% is due to chemists?

Environmental pollutants and hazardous are major issues through which the modern world is suffering. Among all the environmental pollutants and hazardous materials how much contribution is from...

31 December 2020 6,039 3 View

What are the role of microbes for green revolution or modern advancement in science ?

We all know the role of microbes its miniature of the miracle how it can be , used in revolutionizing science in various fields.

23 December 2020 6,220 10 View

What is your opinion of "emotional intelligence" as a modern management tool?

Dear friend, what is your opinion of "emotional intelligence" as a modern management tool? Are there positive practices in your countries and in your institutions with its study and implementation?

14 December 2020 3,867 90 View

Where should I be looking for sources on the history of sharecropping in the United States?

08 November 2020 368 9 View

I need your advice about a PhD dissertation topic on Modern English Novel ?

I am studying Modern English Novel, spcifically postmodern and postcolonial novel. I am very indecisive about determining a topic. Is there anybody recommending me about a topic relating with...

05 November 2020 6,434 9 View

Does anyone have good examples of how aesthetic standards have influenced creative design in building structures?

My research is looking at the influence of creative design and technical standards on the development of architecture during a period of history. This has relevance to the modern day in terms of a...

05 November 2020 5,214 6 View

Which methods (or modern programms) are used to assess the biodiversity of highest vascular plants?

27 October 2020 240 3 View

What are Extensors of space and time in Generalized Thermodynamics?

Generalized Thermodynamics, which formulates the most universals laws of nature, assumes separation of all fields variables into conservable 'extensors' E and 'intensials' P linked by energy dU =...

20 October 2020 8,117 4 View

How the plato's curriculum theory satisfy the modern education pedagogy????

If yes or no justify

02 October 2020 3,395 3 View