Why No plasmid from Y2H positive colonies?

05 May 2020 0 293 Report

I have done Y2H screening with normalized human cDNA library with protein of interest. I have got some positive colonies with blue colour on Leu, Trp, His, Ade dropout + Aureobasidin A and X alpha Gal selection plate then I tried to isolate plasmid from those positive colonies but I am not getting the plasmids. I tried different plasmid isolation protocols but nothing is working. What could be the problem? Anyone faced this? Suggestions please.

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