Can one simply dry PCR products in air?

Hello all! Hope you are doing fine. For the needs of a library preparation (Illumina sequencing of PCR products basically), I need to dry 100ng of each PCR product and then reconstitute it in...

30 November 2020 8,246 11 View

How would you interpret this difractograms?

Currently I'm working with Halloysite and Loading it with 2 different essential oils (One is apolar and the other one is polar). I did the DRX analisys but can't undertand whats going on, can...

09 June 2019 8,496 3 View

Bandwidth selection and number of regressors?

Hello all, I am running a Geographically Weighted Regression and calibrating the bandwidth of my models using GW.model R functions. After some tests and playing around with the data, I...

16 May 2019 3,652 1 View

Multiple choice feeding experiments - Can anyone help me decide on my statistical approach?

I performed a multiple choice experiment to assess the food preference of the sea urchin Paracentrotus lividus in relation to 5 different food items. I have 15 replicates, each represented by a...

03 February 2016 8,060 4 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

No title

22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

SPSS: A scale with multiple items (7-point Likert) across 4 test conditions. How can I correctly summarize it?

Hello, I am currently writing my master thesis where I have a 2x2 factorial design with one downstream variable (2 independent and 2 dependent variables). I need to conduct a multivariate...

11 February 2021 6,014 4 View

Sample preparation for ceramics i.e. iron silicate (fayalite), iron oxides, alumina, spinel mineral (hercynite)?

Recommendations in preparing ceramics for nanoindentation and in the process minimize introducing stresses from sample preparation i.e. polishing stage.

02 February 2021 6,211 3 View

Protocol for bacterial samples preparation for Scanning Electron Microscopy ?

I want to prepare bacterial samples to observe the bacterial cell lysis by bacteriophage protein (endolysin) through scanning electron microscopy. Please, anyone can share the protocol of...

01 February 2021 1,173 3 View

Sample preparation for SEM observation?

hello RG community, I'm looking for Sample preparation protocole for microbiel cells SEM observation. thank you

01 February 2021 7,355 4 View