3-way Robust ANOVA: post-hoc test in R?

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Distinguishing between human activated and exhausted lymphocytes T?

Any suggestions on how to distinguish between activated and exhausted human lymphocytes T CD8 with flow cytometry? It is known that exhausted lymphocytes T have higher expression of inhibitory...

28 December 2020 255 3 View

Can one simply dry PCR products in air?

Hello all! Hope you are doing fine. For the needs of a library preparation (Illumina sequencing of PCR products basically), I need to dry 100ng of each PCR product and then reconstitute it in...

30 November 2020 8,246 11 View

Is this conference a scum?

Hi, can anyone help me to understand if this conference about phytoremediation is a scum? https://waset.org/phytoremediation-phytotechnologies-and-management-conference-in-july-2020-in-prague...

15 January 2020 8,841 3 View

How would you interpret this difractograms?

Currently I'm working with Halloysite and Loading it with 2 different essential oils (One is apolar and the other one is polar). I did the DRX analisys but can't undertand whats going on, can...

09 June 2019 8,496 3 View

Bandwidth selection and number of regressors?

Hello all, I am running a Geographically Weighted Regression and calibrating the bandwidth of my models using GW.model R functions. After some tests and playing around with the data, I...

16 May 2019 3,652 1 View

Help with lentiviral vector?

Hi everyone, I am trying to produce eGFP lentiviral vector by transducting HEK 293T with calcium phosphate method. I transduce cells when they are at 70 % confluent (following this method...

14 October 2018 2,351 4 View

Cross-sex genetic correlation

Hi! We are trying to estimate body mass (W) heritability and cross-sex genetic correlation using MCMCglmm. Our data matrix consists of three columns: ID, sex, and W. Body mass data is NOT...

25 July 2017 6,702 1 View

Which are the best melanoma cell lines?

Hello, I'm working on melanoma and I'm searching the best melanoma cell lines (easy to culture) recapitulating all the steps of melanoma growth: Radial growth-phase cell line, vertical...

05 June 2017 8,061 2 View

I would like to extracte cell membrane from frozen buffy coat. Can anyone suggest a reliable protocol?

Hello everyone, I need to isolate cell membrane of leukocytes and platelet, from frozen buffy coat. Any suggestion?

20 September 2016 5,749 1 View

Fibronectin antibody clone FBN11?

I wish to stain fibronectin in 3D tumor spheroids made with human cancer cells and fibroblasts. do you have a good antibody to suggest? Have you ever used the references MA5-11981 from...

30 June 2016 1,973 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View