hello every one,
Hope reading my question will all be in good health, can some one tell me why my seeds not germinate? actually i planted my alfalfa seeds on 12 June 2018 in sole cropping and inter-cropping with maize but the maize are now at 4-5 leaves stage and the alfalfa are not yet germinate, after two weeks then i reseeded alfalfa but again after three days did not notice any sign of germination could you please help me what are the key problems and their solutions.
Thank you
thank you @ Ajit kumar Roy, its a greenhouse experiment i sow the seeds in pots
Seeds of alfalfa are usually dormant, they have hard testas, so they need hot water treatment before sowing and germination.
you must check the viability of alfalfa seeds. Also they are dormant. ISTA rules recommends prechilling to break dormancy.
Please double check germination on Petri dish first. However, you didn't mention from where you collect your seeds. Is seed quality and source okay?
Intact seeds of Cistaceae , Malvaceae, Fabaceae and Convolvulaceae species generally have physical dormancy. The treatments such as mechanical scarification, acid scarification and dry/wet heat can be used to break physical dormancy.
For more details, see below articles.
https://www.sciencedirect.com/science/article/pii/B9780124166776000068
https://onlinelibrary.wiley.com/doi/full/10.1046/j.1442-1984.2000.00034.x
https://academic.oup.com/aob/article/105/4/627/191382
I agree with Serter. In most cases, it is sufficient to priming the seeds in warm water (room temperature) for 24 h. Besides it is mostly better to check germination of seeds on Petri dishes before starting the experiment. You should use certified seed.
maybe the tough seed shell, you can presoak the seeds with water for hours and sow
Dear Mr. Jamal Nasar,
I agreed with others, your seed are dormant. i suggest you, first check your seed viability by Tetrazolium test (TTC test for viability) and if your seeds confirm the viability then you should go for any of above dormancy breaking method.
thanks to all but can i reseeded the seeds now after dormancy does this time is ok?
Dear Jamal Nasar ;
firstly , I checked your proposal it is perfect .
secondly if you mean germination in lab , the collagenous referred to good possibilities . but if the germination is in field conditions , you have to check the ecological conditions of each of the combination plants ,they must be in a line , check it in your area , if they are not compatible change the alfalfa with other similar plant as medicago or another type , take care of your design , you might in need to replant all of your crops and start from the beginning if your experimental design is about instantaneous coexisting of the components , otherwise no need for replanting if you are studding rail-cropping .
Dear;
The answer Dear Bozena Sera is true,
Seeds of alfalfa are usually dormant,so for germination in pot pre-treatment needed before sowing.
I feel that the Alfalfa seeds are upset with you. They asked you for chocolates and you gave them soil. Well, now there is one way to go about it. That is use MS medium for seed germination and see if the seeds are germinating there. If yes just give the seeds a heat Shock by immersing in boiling water for 15 seconds and then in water having temperature of 8-10C for 30 seconds. If these seeds are not germinating in MS medium then go for new seed stock.
maybe seed hardnes
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