Hello everyone
I'm working on biodegradation of a toxic substance with pure culture and I'm adapting my culture to the substance by using stepwise subculturing. A day after one of subcultures I suddenly realized that my culture had entered stationary phase and this was very strange because the conditions was as same as the last culture and the amount of the toxic substance was the same. The interesting thing was that I had a culture without the toxic substance and that one had entered stationary phase too, after observing the conditions I waited for another 24 hours but nothing changed, so I decided to dump the subculture and do a new one. Does anyone has the same experience to explain the reason of this happening for me ?
Same situation I got in my Work more than three times. But next time I desired why we not use the cultures which are grown between 6-8 Hrs?.I applied this one and got good result.So my suggestion is try this.
Sajjad Ashtari Larki
Hi
Could you please specify your microorganism?
Sajjad Ashtari Larki In general, biodegradation medium is not enriched and this strain may be faced with some stress in the medium. It is recommended to renew your source culture in an enriched medium and then transfer it to biodegradation medium.
Hi,
1. Depletion of growth medium.
2. The growth and activities of microorganism generate some metabolites within the medium which may exhibit deleterious effects on them (bacteria).
Most times, nutrients and minerals required for growth are optimally enriched in Environment where organisms are collected from. The culture medium used in the laboratory may deplete the growth of the organism due to insufficient growth nutrients and environmental factors i.e the organism might be aerobic and culturing them anaerobically may deplete their growth..
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