I think it is a normal problem with a newbie as me. My solvent system is n-hexan:DMC:MeOH. I use silicagel fsp 300-400 and run the column at 35 degrees (room temperature in my country). What do you think is the most factor ? Thanks
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View
What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.
23 July 2024 4,800 1 View
Dear all, May I ask a question about indexed by Scopus. 1 month ago, I have published my paper in the Journal name: Challenges in Sustainability in 20/05/2024. However, this Journal has changed...
06 July 2024 6,734 2 View
Dear Colleagues, I am currently formulating oil in glycerin/water nanoemulsionn of Thymol and Eugenol, using cationic surfactant. After phase diagram, I got trouble that the clear and...
03 July 2024 4,200 2 View
How to calculate Module Young from compressive strength and strain curve
29 June 2024 817 3 View
I have been working in Hanoi University of Public Health. I would like to know where and how I can buy AMOS software?
28 June 2024 6,593 1 View
When choosing the receptor model (rigid or flexible) in molecular docking, does it depend on the designed ligands' characteristics or anything else? For instance: + With the ligand A, the protein...
25 June 2024 2,716 0 View
I prepare Mg doped Hydroxyapatite by wet preipitation but (Ca + Mg)/P ratio is higher than 1,82. CaO peak cannot be detected in XRD spectrum
23 June 2024 8,970 19 View
I follow the protocol in this paper "Computational protein–ligand docking and virtual drug screening with the AutoDock suite" for the "Flexible Docking". Everything is smooth until I write the...
22 June 2024 3,089 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I want to know more about diamond ore deposits in world.
08 August 2024 1,514 0 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
to identify themes in question with APA style references
07 August 2024 2,239 5 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View