Good morning, I have a question and I hope someone can help me. I have as inoculum chlorella vulgaris, with photoperiod of 12/12 hours, shaker agitation up to a volume of 500 mL and agitation provided by fish tank pump for volumes of 1 L and up.
I have observed the formation of a foam on the top of my crops, which I associate to a bacteria producing it but I have not been able to control that, any recommendations?
Also in the upper part of my containers it is forming like a green slime where my microalgae are staying and although I try to homogenize them they do not unite. Could this be associated with
I think it is because of cellular death and it is necessary to add some fresh medium culture to it. check the aerating system and make sure the culture is aerated well.
It may due to the saponins produced by Chlorella. Generally they exhibits high phytochemical content especially n log phase. please subculture the same and if the property reappear go for saponin test
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