In our experiment, the top of our ladder indicates 12,000bp, and in subsequent lanes we have samples where the band shows up slightly above the top of the ladder (however we know for a fact that the bands are less than 12,000bp). Why might this have happened? Does it have something to do with the dye front?
I did not personally perform the lab as the course is being offered online due to the current circumstances, so I might not be able to answer very specific questions about how the experiment was performed. In fact, it is my job to interpret the results and guess why the results from the experiment turned out how they did.
Do you know anything about the nature of the nucleic acids run on the gel or how they were prepared? Was it a plasmid? Is it possible that the plasmid was concatenated (interlinked like the links on a chain)?
Generally supercoiled plasmid migrates much Slower than the linear form. If you used plasmid, this could be the reason.
Perhaps you have genomic dna in your sample or some of your dna is bound to protein so running at a large size
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