To be developed.
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....
14 January 2021 6,326 2 View
I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...
04 January 2021 3,591 3 View
I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan
01 January 2021 5,798 3 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
27 December 2020 3,688 3 View
Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...
01 December 2020 1,178 3 View
Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...
08 November 2020 9,100 3 View
Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.
01 November 2020 8,869 2 View
22 October 2020 9,354 4 View
I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...
10 October 2020 1,830 3 View
Hi, We all know the dispersion relation for an ideal graphene in hexagonal lattice. Since graphene is polycrystalline by nature, assuming a perfect graphene crystals with average size La, nm...
24 January 2021 4,747 2 View
Good day! I am Zachary Origenes, a mechanical engineering student in the Philippines and I am currently doing my thesis which involves validating my simulation model of an in-pipe inspection robot...
17 December 2020 4,431 1 View
In 2015, I upload a working paper that is now published in another version (publication in 2020). Between these two version, authorship changed. Is it possible to remove the following items (the...
23 November 2020 604 2 View
I have tried different pretrained networks with Adam optimizer, and obtained high performance. However, when I tried it with Alex network, the performance was poor. Could you please help me...
28 September 2020 5,621 1 View
I am using google colab to train a model for sign language recognition using this dataset: https://www.kaggle.com/feronial/turkish-sign-languagefinger-spelling I tried different architecture...
08 May 2020 4,098 5 View
I am currently working on Co-simulation of ADAMS-simulink environment. After modelling full vehicle in MSC ADAMS, I need to create state variables to define them as input and output variables. The...
07 March 2020 4,835 4 View
This is not a question but a 'cut and paste' that the computer I'm using right now decided was a question. So going with the 'flow' so to speak I will try to turn this 'cut and paste' into a...
28 December 2019 6,962 1 View
01 November 2019 9,340 1 View
This question is for Principal Investigators. How long should we retain old physical laboratory notebooks from our former students, postdocs, and technicians? This is assuming that all data have...
08 September 2019 1,653 3 View
04 September 2019 5,930 3 View
