Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

Protocol for in vitro cytokine stimulation of immunized CD8+ T cells?

Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...

02 December 2020 9,413 3 View

Do I need to run a factor analysis on all the items used in the SEM to solve the multicollinearity issues?

Respected researchers, I conducted a SEM including 5 variables (two independent variables , two mediators, and a dependent variables), the correlations between these variables range vary from...

01 December 2020 1,338 5 View

How to extract RNA from small number (100-150 at most) of sorted cells?

I'm trying to test expression of a gene in sorted cells. I plan to extract RNA from sorted cells and to do RT-PCR (reverse transcription PCR). The problem is that from sorting, I can only collect...

21 November 2020 5,467 2 View

After place the crudes on an column spectroscopy (sorry, column chromotograph), is it approprate to touch or move the crude sample anymore?

As the title has asked. For instence, using metal stick or long glass tube to get rid of the bubbles in columns.

05 November 2020 5,946 4 View

B cell isolation from mice spleen using Meltinyi negative kit?

Hi, guys, Do you have problem using B cell negative selection kit form Meltinyi? Sometime the B cell is pure, CD19+>95%, but sometimes it lower than 90%.

19 October 2020 5,160 3 View

How to separate exosome and subcellular organelle (eg. ER) of mouse liver?

I want to do tissue subcellular fractionation of mouse liver. According to the method, the speed to separate ER is over 200,000g for 120 min (Song et al., 2006). However, liver exosome isolation...

12 October 2020 4,929 3 View

How to set up rheological model (CA and CAM) ?

how to set up asphalt rheological model (CA and CAM) ? How to build EXCEL?

23 September 2020 7,692 2 View

Should 1:1 binding model be used for antibody binding in biacore(SPR)?

Hi anyone experienced in studying antibody-antigen binding with biacore (SPR) know should 1:1 binding model be used in kinetics and affinity analysis? Or should we use 1:2(antibody:antigen)? Thanks!

26 August 2020 4,130 3 View

How can we deal with the impacts of water management (e.g. drainage) when doing long-term hydrology and water quality modelling?

The anthropogenic pratices are getting common these days and many catchments experience water managements e.g., drainage, which has huge impacts on both discharge and water quality dynamics. So...

13 August 2020 1,801 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View