As a basic of molecular biology, G-C base pairs have 3 hydrogen bonds, while A-T base pairs have two. Therefore, double-stranded DNA with a higher number of G-C base pairs will be more strongly bonded together, more stable, and will have a higher melting temperature. Whereas, double-stranded DNA with a higher number of A-T base pair will be weakly bonded as compare to higher G-C content and less stable with low melting temperature comparably.
The G-C base pairs have 3 hydrogen bonds as compared to A -T base pairs which has 2 hydrogen bonds. Therefore, double stranded DNA with a higher melting point of G-C base pairs will have a higher melting temperature,more stable and strongly bonded as compared to double stranded DNA with a higher number of A-T base pairs will less stable with a lower melting temperature .
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