I´m trying to culture B. japonicum USDA 110 (aka. B. diazoefficiens) under chemolithoautotrophic conditions in minimal saline medium. I found in the literature that this strain requires the supplementation with vitamins B, inositol and potassium iodide (KI).
I understand that under this conditions the bacteria could not synthesize de novo the vitamins and the inositol (probably needed for the biosynthesis of phospholipids?)...
But why is necessary to add KI? And how this element is used by the bacteria?
Dear Pablo,
Most minimal salt media used to growth B. japonicum under a chemolithotrophic condition have no KI.For exemples, see:
HM (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC444536/pdf/aac00351-0048.pdf); H2-uptake agar (https://www.jstage.jst.go.jp/article/jsme2/25/3/25_ME10124/_pdf); or Taylor medium (https://www.ncbi.nlm.nih.gov/pubmed/20173070).
KI is sometime used as a disinfectant.
Kind regards,
103
Hi Pablo
Many useful references in attachment 1
https://collective.chem.cmu.edu/curriculum/labtech/labtech4_dev.php
2
https://www.arpnjournals.com/jabs/research_papers/rp_2007/jabs_0507_50.pdf
3
Article RESPONSE OF ARBUSCULAR MYCORRHIZAL FUNGI AND BRADYRHIZOBIUM ...
5
http://sites.leeschools.net/RoxannaMB/Shared%20Documents/AP%20Biology/Syllabus%20and%20Intro/Mader%20lab%20manual.pdf
6
http://www.jnsbm.org/article.asp?issn=0976-9668;year=2014;volume=5;issue=2;spage=437;epage=444;aulast=Deivanai
7
http://aciar.gov.au/files/node/14200/mn147_practical_methods_for_the_quality_control_o_13633.pdf
8
https://www.imoa.info/HSE/environmental_data/environment/biosphere.php
10
https://link.springer.com/content/pdf/10.1007%2F978-3-319-06542-7.pdf
11
https://www.jove.com/visualize/abstract/20016675/soybean-lectin-enhances-biofilm-formation-bradyrhizobium-japonicum
12
https://www.stii.dost.gov.ph/images/jdownloads/pdf_files/2015/PSTA_July-Dec2015.pdf
15
https://aem.asm.org/content/78/17/6327.full?related-urls=yes&legid=aem;78/17/6327
17
https://www.academia.edu/8906832/Future_of_Idlis_A_Scientific_Assessment_A_Study_on_South_India_s_Most_Preferred_Breakfast_Cereal_
Thank you all for the help. I will take a moment to read all the references.
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