G-quadruplex
I am planning to isolate cells from frozen xenograft tissues (stored in liquid nitrogen). The tissues are stored in 10% DMSO, 30%FBS and 60% RPMI with Penn/Strep. These tissues were frozen with a...
14 December 2020 3,910 1 View
Hello everyone, I am doing an experiment in which, the plan of work is to electroporate a clone (plasmid form) into chicken spermatozoa, which will be used further for artificial insemination....
07 September 2020 7,855 2 View
I am trying to CG relax a amorphous GeSe system with B3LYP and single K-point (gamma). Even though I have a 250 atom model, the calculations seem to be extremely slow. Is there a way to speed up...
23 August 2020 8,848 4 View
I have a UPLC system and XBridge C18 ( 3x150mm, 3.5 um). I am running short of time and cant procure a UPLC compatible column for the time being.
15 December 2019 8,433 12 View
I need a data set of dorsal hand vein images for experimental purpose.
07 October 2019 1,438 1 View
HI, I am finding difficulty with the solubility of Platinum (II) chloride. I wanted to prepare PtCl2 in water for electrochemical application.
21 August 2019 5,444 3 View
We have derived from certain astrometric and cosmological observations how physical constants evolve in time (https://doi.org/10.3390/galaxies7020055). The theoretical basis used is rather...
25 May 2019 8,899 3 View
Hi everyone, I am looking forward for a solution to my problem. I want to attach an enzyme covalently on gold nanoparticle surface. If i follow EDC-NHS chemistry, the enzyme-nanoparticle complex...
14 April 2019 7,238 2 View
Tartaric acid content by HPLC
09 April 2019 3,675 10 View
washed the column in the reverse direction also but the same problem persists. when flow rate is reduced to 0.8mL/min from 1 ml/min...the pressure reduced to 2800 psi apprx. what more can be done...
04 January 2019 7,935 13 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View
I have a DNA sequence which has predictability to form G quadruplex . I need a software to generate a pdb of it which will form G quadruplex. I am using open Babel but I am not able to set the...
29 January 2021 6,190 4 View
When conducting a simple linear regression analysis with one categorical predictor variable and power projected at .80, G*Power suggests a minimum sample size of n = 55. Now, assume that the...
10 January 2021 6,317 6 View
Hello, I am trying to mark cells undergoing proliferation in the brain of adult zebrafish (Danio rerio). I did immunohistochemistry on sections using the Anti-PCNA antibody [PC10] (ab29) with...
14 December 2020 4,989 1 View
Hello, How would you estimate the required sample size for a 2x4 ANOVA using G-Power based on previous research. I am particularly struggling to understand how to define 'number of groups' and...
22 November 2020 9,972 2 View
I'm conducting a correlational study about "is there a relationship between depression, acceptance and quality of life among spinal cord injury patient?" But I am having an issue determining the...
06 November 2020 9,798 10 View
30 October 2020 961 3 View
Hello! I am in the process of writing my dissertation proposal and need to figure out sample size (I will be using G*Power). To measure my DV, I am using a survey that has 4 subscales each with a...
29 October 2020 8,609 2 View
I'm trying to perform quad-IF staining on frozen tissue sections on a fluorescent widefield (axioplan) microscope. I was wondering what people might suggest for colour/strategy for a co-IF...
13 October 2020 6,863 4 View
Guanine-rich DNA sequences can fold into four-stranded, noncanonical secondary structures called G-quadruplexes (G4s). G4s were initially considered a structural curiosity, but recent evidence...
27 September 2020 9,105 6 View
