AS the cd4+ T cells of OT-II mice are specific for chicken ovalbumin 323-339 peptide,OVA protein plus Aluminum hydroxide adjuvant can lead to the specific proliferation of Th cells，why some articles also plus LPS to induce immune? Does LPS induce the non-specific proliferation of Th cells?
Hello Rui Zhao
LPS induces the activation of dendritic cells throughout the engagement of toll-like receptor 4 (TLR4). LPS-activated dendritic cells show increased capacity to process and present pathogen-derived antigens to activate naïve T cells. The dendritic cells which are antigen-presenting cells communicate with T cells both directly and indirectly. These dendritic cells require OVA+LPS in vivo to activate naive OT-II T cells ex vivo. Therefore, you will come across in some studies that LPS is used as an adjuvant when immunizing OT-II mice with OVA.
Best.
Malcolm Nobre Thank you very much, I want to ask you another question about OT-II mice. I found some articel use NP-OVA to immunize OT-II mice. As 4-Hydroxy-3-nitrophenylacetyl is a hapten, compared with OVA alone, does NP-OVA will induce stronger immunity to activate T cell. Thank you again.
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