Optical direct band gap change with substrate, why?

I deposited Zinc thin films on two types of substrate materials, one is ITO and second quartz substrate. When I calculated optical direct band gap there is difference in energy for 0.2-0.4 eV...

03 April 2019 2,467 8 View

How would you extract lipids without using solvents?

I prepared cell culture with phospolipid of interest. However, in extracting it I shouldnt use any kind of solvent. What techniques would you use that dont have liquid phase for extraction of...

04 May 2018 2,895 6 View

How would you ionized Calcium carbonate without using aqueous solution?

most non aqueous inorganic solvent are poisionos, or they contain protons. What do you think is the appropriate method for CaCO3 disolution without using solvent? some crystals are pyroelectric,...

02 March 2018 1,209 0 View

Why Rolling Circle Amplification is not used more in molecular biology?

We didnt even mention it at my BSc and Msc study in molecular biology, I only heard of it in context of sensor material, dna origami and nano DNA senors. Is this technique new? or it was under...

02 March 2018 7,760 2 View

I have a problem obataing GIXRD results of Alluminium thin film. Why?

I used incidence angle of 1 degree. Aluminium and Aluminium Nitride via sputtering method. I didnt annealed substrates, Which I havent done also with Nickel and Copper thin film, but they showed...

01 February 2018 7,569 3 View

Can you suggest reliable in-situ technique for absorption kinetic of pollutant (heavy metals) to nanoparticles?

I would like to know if there is easy, cheap and straightforward way to determined removal rate of pollutants by nanoparticles? I prepared several types of nanoparticles, (bare, polymer coated,...

31 December 2017 3,779 3 View

How to improve spatial resolution in Computed tomography?

Hello, I wanted to know what is wrong with my CT (CAT scan) image. when I do medical imaging everything look normal and clear but after Multi Planar Reformation picture in sagittal and coronal...

09 October 2017 4,520 5 View

Why different stregnt of magnetic field in magnetron sputtering yield to different results, nitridification rate?

05 June 2017 7,175 3 View

Why wustite ( FeO) and nitride (FeN) have same picks in GI XRD?

Iron nitrides thin films were synthesised, and grazing incidence XRD was applied to characterise crystalline state, Picks confirms structure of FeN, but afterwards I used raman to confirm it is...

08 September 2016 3,261 47 View

Why is sorption enhanced in presence of magnetic field?

06 July 2016 4,359 8 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View