I used Enterobacter aerogenes for the extracellular synthesis of AgNPs. After adding the bacterial supernatent to the AgNO3 I exposured the mixture to the heat for short periods 15 seconds three times. Please check the attached photos.
Hello Omar,
the basic reason behind formation of different size and shaped nanoparticles only because of more than one reducing agent present in your reaction solution. Other reason is might be secondary reduction of nanoparticles and this may be possible when you go for different time for reaction, different concentration and changing of heat and time of reaction.
Good day.
The different number of silver nanoparticules are normally assembled and this may lead to different sizes. You can scan the sizes of particules using Atomic force microscopy and see and count the sizes in nm. Mr patil is also right.
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