hello ,
I am working with beta amyloid with the objective to find antiaggregatory compound. Recently , i have performed Tht assay to measure inhibition by the compound. the problem I faced is that even after repeating my experiments i am getting the fluorescence in my test sample more than the control. All my compound are either alkaloid or polyphenols. I am not sure what is the reason for that. I have check the interference of the test compound but they do not seem to have their own fluorescence nor optically active. The control I am using is 44uM peptide in 1% DMSO.
It will be helpful if you could provide some insights.
Have you tested for fluorescence of Tht + test compound without any beta-amyloid? The two compounds could be interacting.
@Adam
yes sir, I did and looking at the intensity i am getting its negligible same as that of negative control where i only put ThT with compound and amyloid beta. So, I dont think my compound is interacting with ThT.
What kind of peptide is your control? are you test with another control peptide or protein control like BSA?
@jaime
the peptide i am using is beta amyloid 1-42. no i am not using any other control. though the intensity of known inhibitor i.e., tannic acid is still showing positive result that is up to 65% inhibition.
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