How do I determine temperature of ethanol during a reflux?

04 February 2021 6,673 4 View

Can I store rotenone working solution in fridge until later use?

For my project, I need to expose zebrafish larvae in petri dish to three concentrations of rotenone (10nM, 30nM and 50nM), and the solutions should be changed daily . My current method was first,...

13 January 2020 4,591 3 View

Does anyone have a good protocol for flagella preparation/purification?

I need a protocol to shear flagella off E.coli O157:H7. I have been using a protocol online that is basically resuspending an overnight culture in a syringe and then, spinning it down to acquire...

29 March 2017 551 2 View

How does one crystallise a protein with the presence of TCEP as the reducing agent, and with it's co-factors (NAD+ and Fe2+)?

I have a protein which I know is stable with the presence of co-factors (NAD+ and Fe2+). Recently i have been trying to purify it with the TCEP as a reducing agent since my protein is known to...

22 November 2016 7,505 2 View

How long can I keep rat's femur/tibia in 10% neutral buffered formalin for?

How long can I keep the bones in NBF before processing for histology and/or immunohistochemistry?

29 August 2016 9,628 2 View

What is the best method to remove soft tissues from a dissected rat's long bone?

I have tried scraping the soft tissues off using a scalpel but the whole procedure was a lengthy one. So I was wondering if anyone could tell me any other quicker way of removing the soft tissues...

22 August 2016 3,507 15 View

How do I check for steric clashes and how to I filter them?

I amrunning DMD modelling to model possible conformations of my proteins. However when I submit my proteins for DMD modelling, it came back to me reporting that it had steric clashes.. How do I...

16 March 2016 2,760 2 View

Do all time series regressions (e.g. multiple linear regression) need stationarity tests?

Do all time series regressions (e.g. multiple linear regression) need stationarity tests?

14 December 2015 1,444 3 View

Why are there no protein expressed even under different expressing conditions?

I am purifying AdhE (96 kDa) protein which is bifunctional and ontains 2 functional domains; (acetaldehyde dehydrogenase and Alcohol dehydrogenase). Before purification, I did a small scale...

27 August 2015 4,381 6 View

How can I validate whether my cross-linking or de-cross linking works?

I am doing Chip-seq, but at the end of the experiment I have obtained a clear DNA background for my negative samples but absolutely no DNA from my tested samples. I think there might be something...

03 August 2014 5,457 7 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View