How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

Transmembrane Protein 3-D Coordinate File Preparation Question?

Hello I hope you are all doing well.:) Several transmembrane Pannexin1 3-D coordinate files have been submitted to the PDB, however, I believe all are missing coordinates in some of the Pannexin1...

11 August 2020 4,875 3 View

What are the best metrics for evaluating the quality of non-spherical clusters?

Hi. I'm dealing with clustering of data were the resulting clusters are, in general, non-spherical. Some of them are not convex. What are the best internal metrics for evaluating these kind of...

27 March 2020 5,076 2 View

How can I separate hematite from bornite in a powder?

17 March 2020 570 3 View

How can I access the total number of integration points present in my model using a vumat in Abaqus ?

Hello, I'm using a vumat in Abaqus and i'm storing data associated to each Integration Point present in my model. For the time being, I scale my array to a given large size, say M(10000). I...

08 January 2020 9,622 3 View

I have tried to clone dcas9 into pET22b vector for the past 1month now. And I do'nt always have any colonies. Anyone cloned dcas9 into pET vectors?

I always have pure insert and vector DNA in the region of 1.7-2.0. I use restriction enzymes sall HF and xhol and perform ligation overnight for 16hrs and then do transformation in 10 beta...

30 September 2019 7,178 2 View

Please, has anyone worked on sgRNA labelling? I am finding it difficult to get protocols to label with alexa dye and Qds.?

How can I label single guided RNA (sgRNA) with Alexa 488 dye and quantum dots (QDs)? I will appreciate any paper recommended or detailed protocols.

30 September 2019 6,873 1 View

How do I stop TEM samples being destroyed under electron beam?

The samples that I make for TEM contain cells. I need them to be 70-90nm (no higher) to be able to see them clearly and do EDX as well. However, They begin to rip and flex under the beam even at...

19 August 2019 2,339 1 View

Correct statistical analysis for intervention study pre and post test?

I have ran a study comparing the Daily Mile and HIIT in children to see which is best for improving cardiovascular endurance (Beep test) and anaerobic power (20m sprint). Scores on both test...

29 April 2019 7,457 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View