No title

02 January 2021 7,417 10 View

What could be the cause of the Glutathione peak migration (LC Dionex, Kintetex EVO C18, water phase) ?

Hello, I need a help from those of you who work on Kinetex EVO C18 with water + 0.2% TFA mobile phase (isocratic). The conditions are constant (temperatures, flow, column, conditioning time)....

16 December 2020 4,582 3 View

How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?

How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?

14 December 2020 7,845 19 View

Macrostudies using sysGMM?

Dear all, Could you recommend macroeconomic studies (hopefully published in good journals) that use system GMM or Arellano-Bond estimators? Thanks a lot!

10 December 2020 5,130 4 View

What do you think about the article in Scientific American that nobody knows how planes lift off and fly?

https://www.scientificamerican.com/article/no-one-can-explain-why-planes-stay-in-the-air/

07 December 2020 4,404 19 View

No title

07 December 2020 9,722 12 View

Why my Nyquist plot does not intersect the x-axis?

I have been measuring the conductivity of the biopolymer membrane by using the 2-electrodes connection and sometimes the plot intersects the a-axis and other times doesn't. The membrane was...

03 December 2020 8,471 1 View

GPower for Three-Way ANOVA analysis?

I want to explore if the amount of time (usage) spent on the Social Media IV1, with two levels , one is light and the other is heavy use, Iv2, body image with negative and positive levels, Iv3...

17 November 2020 4,405 3 View

DNA extraction from bacterial culture?

Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...

06 October 2020 6,890 8 View

Is there an equation connecting wave function and entropy of the quantum system?

Is there an equation connecting the wave function and the entropy of the quantum system?

27 July 2020 931 13 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

What is the best way to isolate Hydrolysable tannins on Sephadex column?

I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...

18 February 2021 1,455 3 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Can any suggest me good method of GC MS for the separation of Flavonoids?

I prepared the ethyl acetate, ethanol and butanol fraction from the ethanol extract of seed for isolation of Flavonoids. Amongst them ethanol fraction showing shinoda test positive for polar...

13 February 2021 8,263 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

What is the steps sequence in the purification of polyphenols from plant roots?

I started by extracting roots samples (8 samples) with boiling Methanol, after evaporation and drying the yield was about 10g (for each sample) of solid material, now for the purification of...

22 December 2020 9,439 4 View

Why there are fewer aptamer-based biosensors and bioassays in commercialization?

Despite the fact of the tremendous number of research papers in the literature dealing with the usage of aptamers as molecular probes in biosensing, and despite the fact that this technology is...

26 November 2020 1,140 3 View

How can we turn a short peptide having affinity for some compound into whole cell biosensor?

Greetings, I have a short stretch of 20 aa with strong binding affinity for a compound. I am trying to de some whole cell flourescence or visible biosensing technique based on this peptide. Can...

26 November 2020 8,322 3 View