In many bacteria PHA/PHB production often takes place in the late logarithmic and stationary phase because they are not directly growth-associated metabolic products. They are the energy storage materials of the cells producing them. PHA production is a common phenomenon in purple non-sulfur bacteria (PNSB). In PNSBs it is observed that nutrient limitation particularly, nitrogen limitation favors the production of PHA/PHB. The stressed condition inferred due to nitrogen limitation triggers the metabolic enzymes responsible for the production of PHA/PHB. Apparently this is a survival strategy exhibited under stressful conditions. Another important factor is that it also increases the C/N ratio which also favors PHA/PHB formation. Some discussions on the metabolic/genetic regulations of this have been provided in the following article.
"Hydrogen from food processing wastes via photofermentation using Purple Non-sulfur Bacteria (PNSB) – A review"