Why does Lehninger say that the eukaryotic ribosome doesn't have an e-site?
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
Hi, I am very new to Bioinformatics. Recently, I have the project that aims to perform taxonomic anlysis on raw reads from mixed samples taken from environment. For example, we perfromed NGS...
09 June 2024 6,537 3 View
Hi all-- I'm preparing plant root samples for RNAseq and have been investigating some samples that had suspicious bands in their lanes when I ran a 1% non-denaturing RNA QC gel. To spot check...
26 May 2024 7,139 1 View
How do researchers avoid over-amplification of eukaryotic chloroplast 16S rDNA when classifying prokaryotic microbial communities in algal bloom experiments? For example, if I need to amplify...
07 April 2024 3,064 0 View
Hello! I am trying to characterize communities of eukaryotes living in biofilms using V4 region 18s amplicon sequencing data. Working with this type of data is new to me, and very interesting!...
04 April 2024 6,603 1 View
I am trying to purify this eukaryotic protein (mol wt 60 kDa) in Rosetta strain. It is getting expressed in good quantity. However, there is another stubborn host protein (mol wt around 70 kDa)...
01 April 2024 3,961 3 View
I have used a column based kit for isolating the RNA from the insect and plant sample. I got a good quantity of RNA, but in the sample QC for Library preparation, the sample failed due to poor RIN...
06 February 2024 9,409 3 View
Metabolically engineered strains in the sense of overexpression or deletion of specific genes within their metabolic pathways, enabling the transfer of biosynthesis pathways between different...
19 December 2023 6,822 1 View
I a working on a 70S ribosome PDB files that contain RNA chains as well as protein chain. I want to delete of hide all protein chain at once. Is there a specific command or any method?
31 October 2023 3,675 2 View