I have run laemmli gels of casein hydrolysates. nevertheless, a blue line appears in the middle of the gel despite only loading half of the lanes. It is worth mentioning that I let the dye front let it reach the bottom of the gel. Any name or solution for my problem?
Is it ready gell or you cast it yourself?
I faced that problem while doing SDS electrophoresis of milk proteins. Turned out that if I wait too long between filling the pipette and continuing to cast the gell i would have a line.
So solved by having larger volume pipette to have faster casting of gel.
Hope that is the case from your side.
Yours
Ossama Dimassi Greetings and thanks for your response. I did not make myself clear. I made a casein hydrolysate which I used to load it in the gel, the gel is acrylamide and when I do the run it paints that blue line that appears along the gel
This might be due to delay in filling the acrylamide in the cassette or discontinuous filling. While casting the gel there might be a minute gap that led the stain penetrate in appear like a line. If you repeat it cautiously you may not find it again.
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