Hello, I am trying to tissue culture various kinds of plants.
I wonder why the protocol always recommend to cut the leaves & roots of the plants when sterilize them and plant them into the media.
Can I leave them instead of trimming them?
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...
24 July 2024 1,515 0 View
UK's Well-online Science Press sent an invitation to be a co-author and offers a payment of $30 per review.
22 July 2024 1,695 8 View
I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...
20 July 2024 4,951 1 View
I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...
16 July 2024 7,475 1 View
I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...
11 July 2024 7,063 1 View
Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
11 July 2024 4,812 5 View
Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...
11 July 2024 8,115 2 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
Dear people,
24 July 2024 1,252 2 View
I'm currently studying the treatment of sewage wastewater and am interested in understanding the impact of autoclaving on the concentration of heavy metals. Specifically, I would like to know if...
10 June 2024 2,579 4 View
I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...
09 June 2024 9,876 0 View
Hi all-- I'm preparing plant root samples for RNAseq and have been investigating some samples that had suspicious bands in their lanes when I ran a 1% non-denaturing RNA QC gel. To spot check...
26 May 2024 7,139 1 View
hand weeding is time consuming, costly and other mechanical weeding practices are not available here. I am seeking for effect of selective herbicides on root nodulation in mung bean farming....
22 May 2024 4,141 0 View
I searched in literatures and i got the formula root length of treated plant / original root length *100 when i applied this, i got the values higher than 100.
10 May 2024 9,148 2 View
I am preparing an experiment in which I would like to sample roots of sunflowers grown in pots (substrate is soil) in later stages of the plant development. Can anyone tell me, what is the best...
29 April 2024 8,775 1 View