After that, rings were placed under a resting tension of 2 g and equilibrated for 90 min before starting the experimental protocols.
If less or more than 90 min what occurs?
Hi Mudhir,
in case of shorter resting time period the answers to agents such as KCl, ACh and phenylephrine are generally lower. For examle as a consequence of lower answers to phenylephrine you cold not apply ACh to evaluate relaxation and the endothelial functions. Shorter resting time answers are not answers of well equilibrated adopted to new envoironment (organ chamber).
Best Regards
Agreed, this time period is necessary (perhaps a time to go get coffee). 30 mins is likely the bare minimum to obtain proper contraction and relaxation data. Perhaps I am over analyzing your comment, but be sure that the 2g isn't instantaneously applied and is applied over time, unless performing a length tension curve, in which case you'll discover that 2g is an approximation, not an absolute.
Hi Mudhir,
You could also take a look at at my paper.
Article Rapid functional upregulation of vasocontractile endothelin ...
Thank you Gry Skovsted
Please what did you mean by ((t 1½, 4, 7 or 24 h after mounting the arteries
in the myograph baths)) in your paper
Best reagrd
In supplementary materials you can find this figure showing that the vasoconstrictor responses to Sarafotoxin 6c increases when isolated arteries are incubated in PSS buffer in the myograph. The first concentration response curve was performed 1 1/2 hours after normalization, the second ar 4 hours and the third at 7 hours. As you can see, sarafotxin 6c induced vasoconstrictor responses increase during short term incubation.
http://www.sciencedirect.com/science/article/pii/S002432051200080X?via=ihub#f0020
The resting time is depends on to the tissue. We before tested it and most of rat aotic rings were equilibrated aprox. in 1 h. but some of them were not and the contractile response to the spasmogens were affected. The contractions to KCl and Norepinefrine were unstable. The contractions of KCl were tended to increase and the NE was to decrease (it does not reach to a plateau) .
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