How important is Chop as a marker for apoptosis or can cell be happy even under high level of chop at mRNA and protein level?

02 March 2015 2,552 6 View

Does anybody know of ATF6 (Activating transcription factor 6) antibodies that have worked in INS-1 cells for western and immunostaining?

I am planning to look at the expression of ATF6 in INS-1 cells under high glucose or ER stress. There are number of antibodies out there but I want to know if anyone in researchgate has personally...

06 July 2014 1,514 2 View

What is the best housekeeping gene that can be used to study the effect of low and high glucose on the expression of various genes in INS-1 cells?

I am trying to analyze the expression of some genes under low and high glucose treatment in INS-1 cells. Most of the housekeeping genes such as actin or GAPDH seems to change a bit under these two...

02 March 2014 3,836 15 View

What is the reliable and quick method to isolate RNA from 384 well plate?

We are interested in identifying compounds that can either increase or decrease the expression of our gene of interest. We usually seed cells in 384 well plates and then incubate cells with stress...

02 March 2014 8,277 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

How seniors face covid 19 without fear?

Seniors in the world with diabetics and hypertension and heart diseases are millions ,and they are facing covid.therefore we should give them advices and precausions to face covid 19,this...

26 January 2021 8,349 4 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

How to measure CPT1/FAO activity in a rare cell type?

Hello all, I am searching for a way to measure the activity of CPT1 and/or FAO in mouse hematopoietic stem cells, which means I have very little material available to do so. Any...

20 January 2021 8,499 2 View