We use one probe with orange (Alexa555) dye to bind to the centromere of one chromosome and another probe with green (Alexa488) dye to bind to a gene. But in some nuclei we can see 5-10 spots that show both colours. However, in other nuclei in the same slide, there are only 2 orange and 2 green spot separately, as we expect it. What could cause this result?
Steingrimur Stefansson
Hi Rita Hargitai . It could be due to precipitation of the probes or that the signal is so strong that it bleeds through the channels.
0 votes
0 thanks
Rahul Sharma
Hi Rita,
Where do you see the signal? If you see the 5-10 costained spots at the nuclear rim, it would likely be a permeability issue. The probes lack access to the nucleus and stick to the periphery instead.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Zoology
- Ichthyology
- Vertebrates
- Fish
More Rita Hargitai's questions See All
Similar questions and discussions