Rita Hargitai

5 Questions 2 Answers 0 Followers

Questions related from Rita Hargitai

Why do we get signals in both colours in FISH?

We use one probe with orange (Alexa555) dye to bind to the centromere of one chromosome and another probe with green (Alexa488) dye to bind to a gene. But in some nuclei we can see 5-10 spots that...

07 July 2019 6,103 2 View

What could be the reason that we don't get specific signals in FISH, only non-specific signals in the background?

We use FISH with commercial probe and hybridisation buffer and with mouse bone marrow cells. We have interphase and metaphase nuclei as well. We use 72 C for 2 min for co-denaturation, then 24 h...

07 July 2018 3,833 5 View

Can denaturation of slides in FISH (in situ hybridization) be performed with normal formamide (not deionised)?

We have performed the denaturation in FISH with normal formamide (73 C), but it seems that the probes did not attach to the chromosomes, there are only non-specific signals. I wonder whether it...

06 June 2018 2,194 3 View

How to normalise to mtDNA copy number when examining mtDNA deletions?

MtDNA copy number is increasing with treatment, but deletion number seems to decline. Is it correct to normalise only to mtDNA copy number or should I somehow take into account that it increases...

08 August 2017 9,177 5 View

What could be the reason for highly variable Ct value differences in a diluteion series of qPCR?

I performed a qPCR with a dilution series (1:2; 1:4; 1:8; 1:16) to calculate amplification efficiency of the target. My results showed that between two points the difference in Ct values were:...

05 May 2017 7,643 0 View