Hi!

Here is a problem I am facing:

I have substituted eGFP with mCherry in the plasmid pires2-eGFP. I have checked it on HEK cell line, and it was just fine. But now I need to use this plasmid in SH-SY5Y. And I see no fluorescence. I have done it in parallel (different wells and co-nucleofection) with the original pires2-eGFP. Green is nice and bright, no signature of fluorescence in red is present.

I took this mCherry CDS from my other plasmid pcDNA3.1-mCherry. And this plasmid works for both: HEK and SH-SY5Y.

Another colleague of mine is having a similar issue with another construct which works fine in HEK but not in the other cell line (which is not the one I'm working with). She also has mCherry in it. 

What might be the problem?