I extracted an active fraction from bacterial biomass through some chromatography. To get totally water free sample, I applied this sample into freeze drying. This freeze drying sample when I open an open space or contract into air, within 30 seconds, all samples became a liquid, i.e absorb moisture from air. So, should I always keep in air tight place like desiccator? What is the matter? Is there any hygroscopic to the extent that is readily absorbs water from the air or the polar nature of the hydroxyl group causes this phenomenon or others matter because my sample has the ability to absorb moisture directly from the air?
The polar nature of the hydroxyl group causes this . This process is called hygroscopy.
You can use some drying agents in the desiccator that make it a bit better (e.g. Calciumchloride, Phosphorpentoxide, Sulfuric acid or silica gel), you can also keep it in a dry atmosphere (e.g. nitrogen box).
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