After centrifuge in pbmc isolation, it was observed that some portion of plasma moved to ficoll. The ficoll was yellow colored. I am not understanding why did this happen.
When to use B3LYP and CAM B3LYP? I also want to know why it is called B3LYP and CAM B3LYP?
11 November 2023 8,858 3 View
i want to deposit film of polycarbazole over silicon wafer through three electrode electrochemical cell.
30 December 2020 8,748 6 View
let us assume i have parameters to model polymer in atlas. what parameters should be changed to use as nanocomposite of that polymer?
06 October 2016 8,408 1 View
At what temperature annealing should be done?
04 April 2015 4,146 3 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
We have no access of pressure plate apparatus
22 July 2024 3,488 2 View
Hello, I recently started working in a soil testing lab and am in charge of operating a new piece of equipment in the lab. We are using 4g soil to 40ml extraction reagent, shaking the mixture,...
26 June 2024 1,265 3 View
Dear colleagues, does anyone know of a suitable alternative for Centrifuge software (calculating settling time for grain size separation by centrifugation), preferably one running on Windows 10 or...
17 June 2024 7,085 0 View
Hi, I have been using a Q-Sonica Q500 Sonicator to disperse samples on nanocellulose and make emulsions. I have notices black dust like particulates settling out in some samples and on the bottom...
12 June 2024 9,737 6 View
I was wondering if the plasma that is in the top layer after centrifugation can be used to assess proteins, metabolites, hormones, or other molecules, or if the ingredients of the Ficoll alter the...
03 June 2024 1,852 4 View
I am researching on azo dye degradation by bacteria. To analyze FTIR, how should I prepare my samples? Do I need to centrifuge, add anything or subtract anything? I am using minimal salt medium...
01 June 2024 3,846 1 View
We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...
28 May 2024 3,529 1 View
For my project, I need to extract RNA from muscle tissue of EEL. I am trying but the yield is low and Purity not good. The 260:230 ratio is always below 1.50 and 260:280 ratio ranges 1.6-1.86 and...
15 May 2024 3,024 2 View