I extracted RNA, converted it into cDNA. Then, I used Qiagen HotStarTaq Plus Master Mix Kit for PCR. Then I run AGE at 80 Volt, 67 mA in 45 minutes (constant: V). I want to check whether my 4 primers are working or not, so first lane ladder, second till fifth lane samples plus forward reverse primers, and another four lanes of NTC. I did not add loading dye to dna ladder, but I used 1 ul loading dye and resuspend in 5 ul of sample/NTC.

I could see the bands without uv (still none for dna ladder) but with uv there's none.

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