We can't find any nodules. Why? Are we using the wrong methodology? We dig up roots and look for nodules. This is in southern Mozambique.
Hi,
We too often struggle to find nodules, even on putatively N-fixing species. Sometimes they are there, sometimes not, and almost always very small. We developed a new protocol for estimating symbiotic N fixation that may be helpful. Sullivan et al. 2014 in PNAS-USA. you should find a PDF on my RG profile.
Yes, they are often hard to see visually, especially on older trees. Try some young or juvenile trees and sometimes they are much more obvious.
Two answers so far; both very useful. I have had a first read of Sullivan et al. Thank you for that very interesting information. Awaiting more answers.
Collect soil from field in pots and germinate the seed - you should get nodules in young seedlings
Dipjyoti Chakraborty, thank you for the suggestion. Which leguminous tree species did you succeed in seeing nodules by your suggested method? We have been trying it, but so far without success.
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