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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How to calculate the field capacity??

28 December 2020 1,521 2 View

Which software should be used for XRD analysis in case of soils?

I am using XRD test for indentification of minerals and amount of it.

10 December 2020 8,173 4 View

How to improve total RNA concentration after transfecting the cells with the desired delivery system ?

Hello, I have been doing some transfection experiments for certain gene studies and wants to further do gene expression studies using qRT-PCR. Currently, I am using 7*10,000 cells per well for...

08 December 2020 6,981 5 View

What is the index of germination and howa calculate it??

16 November 2020 4,856 8 View

How do I determine the units of polymerase activity for my enzyme DNA Polymerase?

One unit of DNA polymerase activity is conventionally defined as the amount of enzyme that will incorporate 10 nmol of nucleotides during a 30-min incubation. I found out about the...

04 November 2020 8,824 7 View

How to find the coordinate location of shared information in Google Drive?

Is it possible to know the coordinates of User Equipment which sent the data/information/file to another User Equipment on Google drive? Does drive has such features or option to identify the...

23 August 2020 3,315 3 View

Is there any journal who currently accepting the papers on IoT in Agriculture ?

I need information regarding publication of journal papers. The indexing of the journal should be SCI/ SCOPUS or equivalent. The topic of the journal can be IoT or automation in Agriculture or...

30 May 2020 6,794 5 View

Three single phase choke or one three phase choke.. which is better to have in circuit ?

I have 3-4 PWM converters , output is 3 phase 400 V AC 50 Hz. Now I want to connect them in parallel, to increase the output kVA rating. So a decoupling choke is required at the output side to...

30 March 2020 4,525 3 View

Can anyone suggest me a good ligand exchange method for surface modification of nanoparticles ?

I am working on certain nanoparticles where I need to modify their surface with a polymer by Ligand exhcange approach. I came across many papers describing many different methods but I am really...

07 October 2019 5,174 2 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View