I am trying to analyse CSDS by capillary electrophoresis (Beckman PA800 plus) using the protocol Pungor et al 2009 Development of a functional bioassay for arylsulfatase B using the natural substrates of the enzyme.
However my peaks are extremely unresolved see picture attached.
I am following the protocol exactly, can't figure out what is wrong.
Could you provide the exact method details used? Hard to troubleshoot without knowing the actual method. *No reason to expect that a published or reported method run on someone else's system will be the same on yours. Lots of variables and differences between systems, instruments, training and operation.
Peak shape is very poor. Causes include: Sample overload, clogged or contaminated capillary, failure to properly condition cap and many others.
Make sure you are using FRESH buffers which have been filtered.
Does the capillary have a nice clean cut? Was it positioned correctly? Was it washed with mobile phase? Is the injection solution fresh, filtered, weaker than the mobile phase and of the correct concentration?
If the capillary is suspect, then get rid of it, install and condition a new one. They have a finite lifetime and with dirty samples or old buffers they will not last long.
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