Hi experts,

I have cloned the DsRED gene into a plant vector under 35S promoter and used it for direct seed transformation of Chenopodium quinoa. When I checked the potential transformed seedlings (3 days old) for fluorescence under the red filter, I observed fluorescence in both control and potential transformants alike. Also, I can see mild fluorescence under the GFP filter too, even though there is no GFP in my plasmid. Could this be due to some auto-fluorescence? If so, how can I differentiate it from DsRED fluorescence? I could see no fluorescence in the newly formed roots which are white. Thoughts?

thanks in advance

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