snRNA vs microRNA vs siRNA vs piRNA vs snRNA
why ?
Hello, I am trying to optimize an antenna array for MIMO radar. I want to start by using a MATLAB code to display the aperture and the PSF (point spread function) of the array. Could anyone give...
10 November 2020 6,502 1 View
I am doing a research on Skin Cancer Detection. I have read a lot of redundant research papers about the problems which related to the same topic. Therefore, I wish to know the recent research...
04 October 2020 8,628 1 View
Research methodology
12 September 2020 8,410 34 View
I'm seeking to model species mass transfer in water electrolysis, yet I'm confused approaching the study since I'm always facing the fact that all mass transfer theories and models are established...
02 September 2020 2,270 5 View
What is the equation according to which the experimental material is given to the laboratory animal according to its body weight by oral dosage
26 August 2020 5,201 3 View
Hello, I am trying to differentiate HT-29 cell line using Methotrexate (MTX). I came across an old article that suggested treating cells for 3 months in order to reach their confluence state,...
26 April 2020 4,964 1 View
1. How many forms of the virus covid -19 2. Does he have multiple colors 3. I need Image from sources should be intended for publication
03 April 2020 8,173 3 View
Using cathodes to measure the ion concentrations as Cl-, F-, Br-, CN- by reading the M.voltage of water samples.
27 January 2020 7,825 3 View
I want to characterize an enzyme.. In stead of one factor at a time procedure i was wondring if it can be done using response surface methodology... Is there any publications for enzyme have been...
30 December 2019 2,807 2 View
Lets say the grid is at ideal frequency 50 hz then the large load is shut of due to which frequency increases beyond 50 which tends to rotate all turbines faster than their previous speed but at...
03 September 2019 8,563 2 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
23 February 2021 8,969 1 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
