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Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

Hello.. please how can I prepare an emulsion with nile red and nile blue dyes for visualization under fluorescent microscope ?

how can I incorporate the dyes to each phase ? do I have to heat them !!

21 June 2024 9,377 1 View

Is the Journal of Pedagogical Research is a trusted journal to publish in? I check in scopus and it is there but I have concerns if it is predator?

Is the Journal of Pedagogical Research a trusted journal to publish in? I checked in scopus website and scimago and I find it is there with scopus Q2 rank; but I have concerns if it is predator?...

08 June 2024 7,678 2 View

Topics for my PhD in TOPSIS?

I'm considering research topics for my PhD in TOPSIS methodology. Could you suggest some potential titles or areas of study that would be both relevant and impactful?

26 April 2024 602 2 View

Seeking Advice: K-Points and Band Configuration for Phonon Calculations?

I'm currently working on phonon calculations using Phonopy and VASP DFPT approach with a 2x2x1 supercell. I'm seeking guidance on: K-Points Selection: How should I determine the appropriate...

29 February 2024 3,955 0 View

Why may I get a Ct value that is higher for one of my treated samples than my control ( zero hour in a time course study) in RT qPCR??

I'm conducting a time course study on gene expression using RT-q PCR for samples treated with 4 conditions: vehicle, RA agonist, Calcitriol agonist or a combination of both agonists over 6 hours....

26 January 2024 7,921 1 View

How to dilute essential oil before antioxidant assay?

What are the solutes used to dilute essential oil for DPPH, TPC, FRAP and Beta/Carotene Bleaching Assay? Can I simply dilute the essential oil using 1 mg/mL in methanol/dH2O for all assay?...

21 November 2023 2,599 10 View

What style or the behavior of battery with draw compare with solar cell?

with draw the behavior of battery that meaning IV characteristic

09 November 2023 8,991 1 View

What is the possible reason why no emulsion formation in beta carotene/linoleic acid bleaching assay?

The procedure that I used 2 mg beta carotene in 10 mL chloroform with 200 mg Tween 40 and 20 mg linoleic acid. Next, chloroform removal by rotary evaporator and 50 mL of distilled water is added.

08 November 2023 1,890 2 View

What is the standard for Beta carotene/linoleic acid bleaching assay?

What is the standard/positive control that can be used instead of Butylated hydroxytoluene (BHT) or Butylated hudroanisole (BHA)? Sample: Plants’ essential oils

06 November 2023 2,250 8 View

any study on protective factors of care givers of dementia and resilience ?

need to review articles on care giving in dementia and challenges faced

12 August 2024 6,424 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there an alternative to a multinomial regression which allows the DV to be non mutually exclusive?

I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...

06 August 2024 1,752 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

In the situation below, can I apply braun and Clark thematic analysis?

I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).

04 August 2024 8,798 7 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

How can AI-driven personalized learning balance individual instruction with fostering social and collaborative skills?

This question tackles the complex issue of ensuring that while AI can tailor educational experiences to individual needs, it doesn't neglect the equally important aspect of fostering social...

02 August 2024 4,751 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View