How to test PSS/E Raw and Seq files for versions 29 and 30?

Dear Colleagues, I develop a software that export and import Raw and Seq files of the PSS/E. I would like to get some help for testing these files with someone that uses PSS/E for Load Flow and...

25 August 2020 7,104 1 View

Adequate overall psychometric properties?

I have evaluated the structure of a 14-item instrument (n=497) getting the following goodness of fit results (ULS estimator, polychoric correlations): X2 (df) = 201.881(76), p

06 June 2020 4,254 2 View

In Abaqus, how do I "tie" a reference point to a random position in a deformable surface, so that the ref. point moves due to the deformation?

I want to place some sensors on a 2D sample, but if possible, I want to avoid using partitions (to avoid increasing my mesh complexity, as my system is a bit slow). Also, I don't want to create...

16 May 2020 8,264 9 View

Does anyone have reliable information to prepare a noninferiority trial?

Dear all, as you may know, in many clinical trials it is often not ethically acceptable to include a control group with no treatment. One option is to use a wait-list, but in many cases we end up...

18 February 2020 2,914 3 View

Is there any culture media, where one could mimic the infection conditions of S. aureus?

At our lab, we frequently grow Staphylococcus aureus using TSB or Chemically Defined Media. The latter gives us some control on the conditions we want to impose to the organism, but I was...

05 August 2019 1,787 5 View

What kind of filter can be used for aqueous samples for GPC?

I want to analize a sample of water soluble polymer but i want to know the type of filter can be used for my sample

06 July 2019 1,186 3 View

What kind of filter can be used for aqueous samples for GPC?

I want to analize a sample of water soluble polymer but i want to know the type of filter can be used for my sample

30 June 2019 8,194 2 View

How to choose emg signal processing?

I have used two methods to process the same electromyography signal and calculate the maximum amplitude of it (RMS vs smoothing). However, I do not know which of them is the most appropriate,...

12 March 2019 9,524 4 View

Why do I have stop codons after the end of my primer?

22 January 2019 8,021 3 View

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12 December 2018 1,267 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View